Ultraviolet sterilization as the most common form of sterilization biological safety cabinet is widely used, but in the use process is affected by many factors, especially the tube radiation intensity is low and improper application can affect disinfection sterilization effect.The several problems of the application of uv lamp right now to discuss with you.
A, scope of application:
1, ultraviolet rays can kill all kinds of microorganisms, including bacteria breeding, buds, mycobacterium, viruses, fungi, mycoplasma and rickettsial, all who are above the surface of the microbial contamination, water and air are used ultraviolet disinfection.
2, ultraviolet irradiation energy is low, penetration is weak, only can kill microorganisms, direct illuminate to so disinfection disinfection parts must be fully exposed to ultraviolet light.
3, ultraviolet disinfection is the most suitable temperature range from 20 to 40 ℃, the temperature is too low will affect disinfection effect, sterilization time can be appropriately extended, for air sterilization, disinfection environment relative humidity below 80% is good, otherwise should be appropriately extended exposure time.
4, use ultraviolet light to kill is protection of microbial organic matter, should increase the exposure dose.Air and water suspended particles can also affect disinfection effect.
Second, the ultraviolet lamp irradiation intensity of questions:
Biological safety cabinet new ultraviolet lamp tubes is not less than 100 mu w/cm2, 70 uw/cm2 nearly 50 _80 %, control of the ministry of health disinfection requirements of technical specification, the qualified rate of less than 50%.Some unit using ultraviolet germicidal lamp irradiation intensity is less than 70 uw/cm2. Some even less than 40 uw/cm2 still in use. Want to know, each type of microorganism has its specific uv kill dose threshold. The sterilization dose (K) is the light intensity (I) and irradiation time (t), the product of K =. It can be seen from the type of high strength and short time or low intensity light for a long time can get the same sterilizing effect. However, if the uv intensity is less than 40 uw/cm2, the longer exposure time also cannot have the sterilization effect of satisfaction, there's a microbial damage to fix the problem, deliberately in order to improve the light intensity is given priority to.
Three, matters needing attention:
1, dust on the surface of the uv lamp, and oil dirties, prevents ultraviolet penetration, tube cleaning and cleaning, should be paid attention to in using the new tube before use, can use first 75% alcohol sponge to wipe.In the process of using general cleaning once every 2 weeks.Found the tube surface dust, grease, shall, from time to time to wipe, keep clean and transparent tube lights, lest affect the penetration of ultraviolet ray and radiation intensity.
2, use ultraviolet disinfection goods surface, should make light surface by direct exposure to the sun's ultraviolet rays, and should achieve enough exposure dose.
3, uv intensity meter calibration once a year at least, less than 40 uw/cm2, the service life of ultraviolet germicidal lamp shall be not less than 1000 h.Found insufficient strength of the lamp should be replaced immediately.Uv to visible light, low pressure discharge lamp of the purple blue glow as the mercury vapor pressure, while the strength of the mercury vapor pressure and ultraviolet (uv) still has its relevance, but does not directly represent the intensity of ultraviolet.So do not visible purple blue light intensity, intensity of ultraviolet ray.This means that the uv intensity can not be determined with the naked eye.
4, use ultraviolet germicidal lamp should also pay attention to prevent ultraviolet radiation damage problem: mainly to prevent ultraviolet ray to eye, face a radiation damage, especially attention to eye conjunctiva of radiation damage. After radiation injury of the face, eyes red and swollen, tears, and the sting and the electro-optic eye damage is more serious. After three or four days to heal.(one eye conjunctiva for ultraviolet radiation damage, can milk eye drops of choose and employ persons, half an hour at a time, three to four times better). |
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